A multiparametric CAR T cell potency assay that employs real-time cellular impedance and live cell imaging
نویسندگان
چکیده
منابع مشابه
Real-time live imaging of T-cell signaling complex formation.
Protection against infectious diseases is mediated by the immune system (1,2). T lymphocytes are the master coordinators of the immune system, regulating the activation and responses of multiple immune cells (3,4). T-cell activation is dependent on the recognition of specific antigens displayed by antigen presenting cells (APCs). The T-cell antigen receptor (TCR) is specific to each T-cell clon...
متن کاملReal-time cell-impedance sensing assay as an alternative to clonogenic assay in evaluating cancer radiotherapy.
Intrinsic radiosensitivity of normal and tumour tissues has been shown to be an independent prognostic factor for patients' response to radiotherapy. This study compares the real-time cell-impedance sensing (RT-CES) assay with the conventional clonogenic assay in terms of in-vitro radiosensitivity. One objective in this study was to predict in-vivo response to gold nanoparticle (GNP) treatment ...
متن کاملImaging Case: NK/T-Cell Lymphoma, Nasal Type
Peripheral T-cell lymphomas are a group of heterogeneous disorders and according to WHO classification, are categorized into nodal and extranodal forms. NK/T-cell lymphoma, nasal type, is a subtype of extranodal peripheral T-cell lymphoma and commonly presents as a midfacial destructive lesion. This disorder is more prevalent in Asia and South America and has a strong association with Epstein B...
متن کاملReal-time Cellular Impedance Analysis System
The cell impedance analysis technique is a label-free, non-invasive method, which simplifies sample preparation and allows applications requiring unmodified cell retrieval. However, traditional impedance measurement methods suffer from various problems (speed, bandwidth, accuracy) for extracting the cellular impedance information. This thesis proposes an improved system for extracting precise c...
متن کاملA Multiparametric Live-Cell Cytotoxicity analysis using the Operetta
Cytotoxicity is a very complex process affecting multiple pathways. The ability to measure early indicators of toxicity is an essential part of drug discovery. An important approach to the detection of compound toxicity is a multiparametric analysis at the level of individual cells using High Content Analysis (HCA) applications [Abraham et al., 2008]. Such cell-based assays, which assess severa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytotherapy
سال: 2020
ISSN: 1465-3249
DOI: 10.1016/j.jcyt.2020.03.304